Choose language
< Return to main menu
Choose language
Services & Solutions
Services & Solutions
News
Resource
Contact
Career
Peptide CMC
Peptide Discovery
Peptide CMC
Peptide Conjugation
Amino Acid
Case Study
Analytical
API
Drug Product
Analytical
CMC Filing
WuXi TIDES has a dedicated peptide analytical team of scientists with extensive experience and expertise to support all peptide analysis at all development phases. Equipped with state-of-the-art instruments and located in the same site with API process development team, our peptide analytical service will streamline your peptide development with customized, flexible and integrated analytical solution.
Method development and validation
Stability study: forced degradation study, accelerated and long-term stability study under R&D and GMP environment
Purity and Assay, residual solvents, moisture, cation content, anion content, aggregation content, elemental impurities
Microbiology safety: endotoxin for limit and quantitation and bioburden for microbial limit test, microbe ID and strain typing
Amino acid analysis and enantiomeric purity: acid hydrolysis and derivatization followed by LC-MS analysis to accurately evaluate the amino acid ratio and D-isomer impurity amount in peptide sequence
Identity by peptide mapping: enzymatic digestion followed by LC-MS analysis for characterization and confirmation of peptide API
Identity by molecular weight and sequencing: 2D UPLC coupled with Q-TOF and MALDI-TOF/TOF HRMS for accurate MW and sequence determination
Accurately determine the peptide enantiomeric purity was difficult. Peptides with different amino acid isomers have the same physicochemical properties that cannot be analyzed intact; free amino acid does not have good retention for HPLC-UV analysis; peptide contain multiple amino acids that interfere with each other during analysis. We have capability of enantiomeric purity analysis for all 19 isomeric natural amino acids (Asparagine and Aspartic acid, Glutamine and Glutamic acid will be reported together) and Ornithine.
High Performance Liquid Chromatography (HPLC)
High Performance Liquid Chromatography coupled with Mass Spectrometry (HPLC-MS)
Ultra-High Performance Liquid Chromatography (UHPLC - available detectors: UV, CAD, MALS)
Ultra-High Performance Liquid Chromatography coupled with Mass Spectrometry (UHPLC-MS)
2D Ultra-High Performance Liquide Chromatography (2D-UPLC)
Gas Chromatography (GC)
Ion Chromatography (IC)
Thin Layer Chromatography (TLC)
Electrospray Mass Spectrometry (ESI-MS Orbitrap & Q-TOF)
Matrix Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-TOF/TOF)
Tandem Mass Spectrometry Sequencing (Q-TOF, Orbitrap & MALDI-MS/MS)
2D Ultra-High Performance Liquide Chromatography coupled with HRMS (Q-TOF)
Mass Spectrometry Purity and impurity quantitation (Q-TOF)
Gas Chromatography Coupled with Mass Spectrometer (GC-MS)
Infrared Spectroscopy (IR)
UV/VIS Spectroscopy
Tm (UV melting curve)
Circular Dichroism Spectroscopy (CD)
Nuclear Magnetic Resonance Spectroscopy (1H, 13C, 31P & 19F - NMR)
Amino Acid Analysis (AAA)
Elemental Analysis (CHN)
Enantiomeric Purity
Polarimetry
Refractometry
Titration
Turbidimetry
Osmolality
Total Organic Carbon (TOC)
Total Viable Aerobic Counts
Endotoxins (LAL-test)
Heavy Metals
Sulfated Ash
Residue on Ignition
Polydispersity
